Recombinant DNA Technology – Section 1

31. Which of the following pair will produce complementary sticky ends?

A. Eco RI & MspI
B. Msp I & HPA II
C. Sau 3A & Bam HI
D. Mbo I & Sau 3A

Correct Answer: C. Sau 3A & Bam HI

32. A human cDNA library

A. contains DNA for specific human proteins
B. contains DNA for virtually all of the human proteins in vectors
C. cannot be used to obtain human genes because it would be radioactive
D. none of the above

Correct Answer: B. contains DNA for virtually all of the human proteins in vectors

33. What enzyme forms covalent bonds between restriction fragments?

A. DNA primase
B. DNA helicase
C. DNA polymerase
D. DNA ligase

Correct Answer: D. DNA ligase

34. Which of the following can be diagnosed by amniocentesis?

A. Down’s syndrome
B. Cystic Fibrosis
C. Sickle cell anemia
D. All of these

Correct Answer: D. All of these

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