Recombinant DNA Technology – Section 1

16. Which of the following genetic diseases would be amenable to genetic engineering?

A. Down’s syndrome
B. Muscular dystrophy
C. Cystic fibrosis
D. Cri du Chat

Correct Answer: C. Cystic fibrosis

17. X-rays cause

A. the formation of thymine dimers
B. ionization of water in the cell
C. heat
D. none of the above

Correct Answer: B. ionization of water in the cell

18. The order for the construction of a cDNA fragment from mRNA is to

A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligoC, treat with DNA polymerase
B. treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
C. digest with RNase, add G residues to the 3′ end, treat with reverse transcriptase, add G residues to the 3′ end and treat with DNA polymerase
D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligodT and treat with DNA polymerase

Correct Answer: A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligodC, treat with DNA polymerase

19. The TP53 gene of chromosome 17 codes for a protein __________ .

A. that plays a role in the digestive process
B. involved in glucose transport
C. involved in the regulation of the cell cycle
D. that is like a white blood cell protein

Correct Answer: C. involved in the regulation of the cell cycle

20. Why is golden rice pale yellow in color?

A. It is rich in chlorophyll a.
B. It is rich in beta-carotene.
C. It is rich in chlorophyll b.
D. It is rich in phycobilins.

Correct Answer: B. It is rich in beta-carotene.

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