Recombinant DNA – Section 1

1. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

A. southern blotting
B. northern blotting
C. polymerase chain reaction
D. DNA replication

Correct Answer: C. polymerase chain reaction

2. The Southern blotting technique depends on

A. similarities between the sequences of probe DNA and experimental DNA
B. similarities between the sequences of probe RNA and experimental RNA
C. similarities between the sequences of probe protein and experimental protein
D. the molecular mass of proteins

Correct Answer: A. similarities between the sequences of probe DNA and experimental DNA

3. Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

A. BACS
B. expression vectors
C. YACS
D. all of these

Correct Answer: B. expression vectors

4. Agrobacterium tumefaciens is

A. a disease in humans that causes loss of sight
B. a bacterium that can be used to introduce DNA into plants
C. a fungi that is used to produce antibiotics in large amounts
D. a disease in humans that causes loss of weight

Correct Answer: B. a bacterium that can be used to introduce DNA into plants

5. In genetic engineering, a chimera is

A. an enzyme that links DNA molecules
B. a plasmid that contains foreign DNA
C. a virus that infects bacteria
D. a fungi

Correct Answer: B. a plasmid that contains foreign DNA

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