1. Reverse transcriptase from HIV does not
A. uses RNA as a template
B. has a 5′ to 3′ polymerase activity
C. has a 3′- 5′ exonuclease activity
D. requires a primer
2. Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
A. Asn and Gln
B. Cys and Met
C. Lys and Leu
D. Ile and Val
3. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A. positive direction
B. 3′ to 5′ direction
C. 5′ to 3′ direction
D. negative direction
4. The type II restriction endonucleases bind to DNA sites
A. using H-bonds for specificity
B. that are usually palindromic
C. using non-sequence-dependent backbone interactions
D. all of the above
5. The enzyme that joins DNA cuts is called
A. joins
B. ligase
C. DNA phosphorylase
D. reverse transcriptase
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