Polymerase Chain Reaction – Section 1

6. RNA populations can also be used as PCR templates after reverse transcription into

A. c DNA
B. t RNA
C. m DNA
D. r RNA

Correct Answer: A. c DNA

7. The cloning step in PCR/sequencing analysis of microbial communities is necessary for

A. the amplification process
B. preventing contamination by outside DNA
C. separating the different rRNA gene sequences in the mixture
D. none of these

Correct Answer: C. separating the different rRNA gene sequences in the mixture

8. The template for PCR is

A. RNA
B. single stranded DNA
C. double stranded DNA
D. none of these

Correct Answer: C. double stranded DNA

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