Immunological Techniques – Section 1

6. What is the ELISA test intended to measure?

A. Antibody to HIV only
B. Antigen to HIV only
C. Presence of free, circulating virus in the patient
D. Antibodies directed against HLA molecules

Correct Answer: A. Antibody to HIV only

7. Immunoaffinity chromatography can be used in biochemical applications to

A. break down antibody structure
B. purify protein antigen
C. break down antigen and analyze quantitatively
D. none of the above

Correct Answer: B. purify protein antigen

8. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

A. Open circular, super coiled, linear plasmid DNA
B. Linear plasmid, open circular, super coiled plasmid
C. Open circular, super coiled, linear plasmid DNA
D. Open circular, linear plasmid, super coiled plasmid

Correct Answer: B. Linear plasmid, open circular, super coiled plasmid

9. In Rocket Immunodiffusion the length of the rocket is

A. proportional to the amount of antibody placed in each well
B. inversely proportional to the amount of antibody placed in each well
C. inversely proportional to the amount of antigen placed in each well
D. proportional to the amount of antigen placed in each well

Correct Answer: D. proportional to the amount of antigen placed in each well

10. Radial Immunodiffusion is similar to

A. double-diffusion
B. gel diffusion
C. Ouchterloney technique
D. all of these

Correct Answer: A. double-diffusion

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