36. Automated DNA sequencing uses the chain termination method
A. but with an oligonucleotide primer labeled with a fluorescent dye
B. but with a polynucleotide primer labeled with a fluorescent dye
C. with no primer
D. either (b) or (c)
37. Okazaki fragments occur on the __________ and are bonded together by __________
A. Leading strand, polymerase
B. mRNA, anticodons
C. Lagging strand, ligase
D. tRNA, polymerase
38. The RNA primers used to initiate replication in E. coli
A. result in Okazaki fragments on leading strand
B. are removed by Pol I
C. are joined together by DNA ligase
D. are removed by helicase + ATP
39. An enzyme that recognizes foreign DNA, and results in a cut in that DNA is called
A. transposase
B. helicase
C. reverse transcriptase
D. restriction endonuclease
40. Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?
A. 5′ → 3′ polymerase
B. 3′ → 5′ exonuclease
C. 5′ → 3′ exonuclease
D. all of the above
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